Kumasi Asante Kotoko were on the receiving end of a controversial penalty in their 3-1 defeat to Legon Cities at the Baba Yara Stadium.
The decision came about in the 18th minute when referee Selorm Yao Bless pointed to the spot after adjudging that right-back Christopher Nettey had brought down Legon Cities winger Jonah Attuquaye.
But television replays showed showed Attuquaye appearing to have already slipped and Nettey appearing not to touch him.
Two weeks ago, Kotoko were on the beneficiaries of another dubious refereeing decision, when they were awarded a penalty in their 1-0 win against rivals Hearts of Oak. Referee Joseph Paddi was subsequently banned for the rest the season.
The incident has generated huge debate on social media after the video was posted on twitter.